Transgenic Plant Male Sterility

Description:

Summary

Proof of concept for a repressor/restorer male sterility system to produce hybrid seed in Arabidopsis has been achieved. The system can also be used for pollen and seed containment.

The Problem

Hybrid Vigor is characterised by the production of larger plants, tolerance to stress, disease resistance, uniformity and improved yield with plants often being referred to as F1 hybrids. Hybrids can only work in conjunction with pollination control mechanisms. There are three commonly used methods; mechanical (expensive, time consuming, laborious), chemical (transitory and expensive from repeat application of gametocides) and genetic (usually cytoplasmic male sterility but is generally not 100% reliable due to restorer genes and can be particularly problematic for corn, oilseed rape and wheat).

The Science and Innovation

Proof of concept for a repressor/restorer male sterility system to produce hybrid seed in Arabidopsis has been achieved. The system can also be used for pollen and seed containment.  An additional inducible component is incorporated into the system to obtain homozygous female parent lines. The strategy ensures that all female progeny are male sterile (other existing male sterility systems could only produce male sterility in 50% of female progeny).

Homologues of the Myb103 gene occur in canola, rice, wheat, barley, grape, corn, sorghum and populus (tree). The homologues in canola, rice and wheat have been shown to be expressed in anthers. Other transcription factors can also be targeted using this technology provided that they are essential to male fertility. Thus this system could potentially be used for any flowering plant. Recently, male sterile canola has been generated using this system.

The reversible male sterility system is designed so the ‘master’ genes involved in pollen production can be inactivated in the female parents leading to male sterility with restoration in the F1 hybrid plants. Furthermore, the potential incorporation of an inducible activator or repressor in the system means the plants can be sprayed with the inducer at any stage during development to control male fertility.

The Intellectual Property

Transgenic Plant Male Sterility PCT/AU2010/001503

Opportunity:

La Trobe University intends to commercialise the technology by partnering with, or licensing to, a company or companies that combine capabilities in the development of genetically modified plants and in particular, field trials and associated regulatory compliance, with the capacity and end-user relationships required to take this technology to market.

Patent Information:
Category(s):
Biotechnology
For Information, Contact:
Sharon Joseph
IP Manager
La Trobe University
S.Joseph@latrobe.edu.au
Inventors:
Song Li
Roger Parish
Keywords:
Biotech - Agricultural